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| Whole Blood CD45 MicroBeads |
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| Description |
Whole Blood CD45 MicroBeads were developed for rapid positive selection of CD45+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD45 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes, platelets, and their precursor cells. |
| Applications |
Whole Blood MicroBeads allow the fast isolation of CD45+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS™ Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD45+ cells are well-suited for further flow cytometric, functional, or molecular analysis including chimerism analysis after allogeneic stem cell transplantation1.
For more information on MACS® Whole Blood MicroBeads click here. |
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| Figure 1 |
| Leukocytes were separated from whole blood using Whole Blood CD45 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were fluorescently stained with CD45-FITC and CD235a (Glycophorin A)-PE. |
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| Products |
| Whole Blood CD45 MicroBeads, human |
| For research use only |
for 40 mL whole blood
Download data sheet
130-090-872
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| MACS References |
| 1. Köhl et al. (2003) Leukemia 17: 232–336.[2571] |
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