| Description |
| The CD209 (DC-SIGN) MicroBead Kit has been designed for the magnetic separation of human CD209+ cells. CD209 (Dendritic Cell Specific ICAM-3 Grabbing Non-integrin, DC-SIGN) is a type II C-type lectin receptor. It is expressed on DCs in dermal and mucous tissue, in lymphoid tissue such as tonsil, lymph node, and spleen, and on monocyte-derived DCs (Mo-DCs). It is not expressed on DC subsets in peripheral blood, except for a subpopulation of CD14+ cells with DC-like phenotype. DC-SIGN acts as an adhesion receptor to facilitate interactions between DCs and T cells or DCs and endothelial cells. In addition, it serves as an antigen receptor to mediate internalization of ligands for antigen presentation, binding viruses such as HIV, HCMV, or Ebola as well as parasites, bacteria, or yeast. |
| Applications |
| The CD209 (DC-SIGN) MicroBead Kit can be used for positive selection or depletion of cells expressing the human CD209 (DC-SIGN) antigen. |
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| Figure 1 |
| Separation of CD209+ cells from human PBMCs spiked with 1% Mo-DCs using the CD209 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Mo-DCs were generated in vitro with GM-CSF and IL-4 for 7 days. Cells were stained with CD209 (DC-SIGN)-FITC and CD304 (BDCA-4/Neuropilin-1)-PE and analyzed by flow cytometry. |
| Before separation |
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Cells depleted of
CD209 (DC-SIGN)+ cells |
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| Isolated CD209 (DC-SIGN)+ cells |
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