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| Myeloid Dendritic Cell Isolation Kit |
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| Overview |
| The Myeloid Dendritic Cell Isolation Kit has been developed for the concurrent isolation of untouched CD1c (BDCA-1)+ and CD141 (BDCA-3)+ myeloid dendritic cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-MDCs are labeled with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion over a MACS column. Unlabeled MDCs pass through the column.
Downstream applications
MDCs isolated with the Myeloid Dendritic Cell Isolation Kit are used when direct labeling of surface molecules is undesired. |
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| MDCs were isolated from human PBMCs using the Myeloid Dendritic Cell Isolation Kit. Cells were fluorescently stained with CD141 (BDCA- 3)-FITC, CD20-PE, CD14-VioBlue, and CD1c (BDCA-1)-APC and analyzed by flow cytometry. Exclusion of CD1c+CD20+ B cells and CD14+ monocytes was performed. Shown are CD1c (BDCA-1) and CD141 (BDCA-3) stainings of total cells before and after separation. |
| Before separation |
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| After separation |
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| Favorites / Prices |
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| Details |
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| Products |
| Myeloid Dendritic Cell Isolation Kit, human |
- for 2×109 total cells
Download datasheet
130-094-487
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| References |
| 1. Dzionek et al. (2000) J. Immunol. 165: 6037-6046. |
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