| Description |
The CD303 (BDCA-2) MicroBead Kit was developed for the isolation of plasmacytoid dendritic cells (PDCs) by indirect magnetic labeling with CD303 (BDCA-2)-Biotin and Anti-Biotin MicroBeads. The kit includes FcR Blocking Reagent, CD303 (BDCA-2)-Biotin, and Anti-Biotin MicroBeads.
Like CD304 (BDCA-4/Neuropilin-1), the CD303 (BDCA-2) antigen1–4 is a specific marker for CD11c– CD123+ PDCs in blood1,3 and bone marrow5. Additionally, CD303 (BDCA-2) expression is strictly confined to PDCs in tissue, e.g., tonsils or lymph nodes.3,5
CD303 (BDCA-2) is a novel type II transmembrane C-type lectin.3,5 Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits IFN type I production, which is induced in PDCs by, e.g., influenza virus, CpG oligonucleotides, or serum of systemic lupus erythematosus patients.3,5,7 Remarkably, PDCs can uptake ligand via CD303 (BDCA-2) and process and present ligand to T cells.1,3
CD303 (BDCA-2)+ dendritic cells show a plasmacytoid morphology, express the pre-T cell receptor α-chain and are CD4+, CD45RA+, CD304 (BDCA-4/Neuropilin-1)+, CD141 (BDCA-3)dim, CD1c (BDCA-1)–, and CD2–. They lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and express neither myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FceRI.1
Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells.
For evaluation of MACS® Separations using the CD303 (BDCA-2) MicroBead Kit, staining with CD304 (BDCA-4/Neuropilin-1) antibodies is recommended.
For information on clinical products for the isolation of PDCs click here. |
| Applications |
The CD303 (BDCA-2) MicroBead Kit is the most appropriate tool for isolation of PDCs from tissue, whereas the CD304 (BDCA-4/Neuropilin-1) MicroBead Kit is recommended for their isolation from blood or bone marrow.
PDCs were isolated for functional analyses and compared with other dendritic cell subsets.1,3 Furthermore, mRNA was isolated from enriched CD303 (BDCA-2)+ cells for expression cloning of CD303 (BDCA-2)3 or for analyses on expression of DCAL-1 by RT-PCR6. The kit can also be used to deplete CD303 (BDCA-2)+ cells from, e.g., tonsillar cell suspensions8.
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