| Description |
The Plasmacytoid Dendritic Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched plasmacytoid dendritic cells (PDCs) from PBMCs. For their isolation, non-PDCs (i.e. T cells, B cells, NK cells, myeloid dendritic cells, monocytes, granulocytes, and erythroid cells) are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Upon subsequent magnetic separation the labeled cells are retained in the column, while the unlabeled PDCs are collected in the flow-through. The isolated PDCs are CD303 (BDCA-2)+, CD304 (BDCA-4/Neuropilin-1)+, CD123+, CD4+, CD45RA+, CD141 (BDCA-3)dim and CD1c (BDCA-1)–, CD2–, lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56), and express neither myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FceRI.
For evaluation of MACS® Separations using the Plasmacytoid Dendritic Cell Isolation Kit, staining with CD303 (BDCA-2) antibodies is recommended. |
| Applications |
| PDCs isolated with the Plasmacytoid Dendritic Cell Isolation Kit are used when direct labeling of surface molecules could interfere with downstream applications, e.g., in studies on the surface molecules themself, their role in cell activation, and their differentiation. The Plasmacytoid Dendritic Cell Isolation Kit can also be used to enrich plasmacytoid dendritic cells from tonsils, if the CD303 (BDCA-2) MicroBead Kit is not suitable. |
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| Figure 1 |
| Isolation of human PDCs from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit,an LD Column, and a MidiMACSâ„¢ Separator. |
| PBMCs before separation |
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| Enriched plasmacytoid dendritic cells |
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| Figure 2 |
| Working scheme for the isolation of untouched plasmacytoid dendritic cells using the Plasmacytoid Dendritic Cell Isolation Kit. |
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