| Overview |
Please notice: This product has been replaced by the Plasmacytoid Dendritic Cell Isolation Kit II, human (# 130-097-415), which has even better performance.
The Plasmacytoid Dendritic Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched human plasmacytoid dendritic cells (PDCs) from PBMCs. |
| Details |
Background information
The isolated PDCs are CD303 (BDCA-2)+, CD304 (BDCA-4/Neuropilin-1)+, CD123+, CD4+, CD45RA+, CD141 (BDCA-3)dim and CD1c (BDCA-1)–, CD2–, lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56), and express neither myeloid markers such as CD13 and CD33, nor Fc receptors such as CD32, CD64, or FcεRI.
Detailed separation procedure
For their depletion, non-PDCs are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Upon subsequent magnetic separation the labeled cells are retained in the column, while highly pure unlabeled PDCs are collected in the flow-through.
Downstream applications
PDCs isolated with the Plasmacytoid Dendritic Cell Isolation Kit are used when direct labeling of surface molecules ist undesired, e.g., in studies on the surface molecules themself, their role in cell activation, and their differentiation. The Plasmacytoid Dendritic Cell Isolation Kit can also be used to enrich plasmacytoid dendritic cells from tonsils, if the CD303 (BDCA-2) MicroBead Kit is not suitable. |
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| Figure 1 |
| Isolation of human PDCs from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit,an LD Column, and a MidiMACS™ Separator. |
| PBMCs before separation |
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| Enriched plasmacytoid dendritic cells |
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| Figure 2 |
| Working scheme for the isolation of untouched plasmacytoid dendritic cells using the Plasmacytoid Dendritic Cell Isolation Kit. |
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