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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
 
 
 
 
 
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Anti-PTK7 (CCK-4) MicroBead Kit
Overview
The Anti-PTK7 (CCK-4) MicroBead Kit has been developed for the isolation of PTK7+ cells from human PBMCs and bone marrow mononuclear cells (BM-MNCs).
Details
Background information
The monoclonal anti-PTK7 antibody 188B recognizes human protein tyrosin kinase-7 (PTK7). PTK7, which is also known as colon carcinoma kinase-4 (CCK-4), is a receptor protein tyrosin kinase (RPTK)-like molecule which contains a catalytically inactive tyrosin kinase domain.1,2 PTK7 mRNA is detected in normal human melanocytes, colon carcinoma cells, as well as lung, liver, pancreas, kidney, and placenta tissue. Recently, using the specific monoclonal antibody 188B, PTK7 was shown to be expressed in blood and bone marrow, on CD303 (BDCA-2)+ plasmacytoid dendritic cells, CD141 (BDCA-3)high type-2 myeloid dendritic cells, and CD34+ hematopoietic progenitor cells (HPCs). PTK7 was detected on early (CD34+ CD133+) and late (CD34+ CD133-) HPCs. In tonsils, PTK7 was also found on some T cells.

Applications
Positive selection or depletion of PTK7 (CCK-4)+ cells from blood, body fluids (e.g. bronchial lavage), or single-cell suspensions of bone marrow or tissue (e.g. lymphoid and tumor tissue). The kit can be used for simultaneous isolation of PDCs, MDC2s, and HPCs from PBMCs or BM-MNCs, or in combination with the CD1c (BDCA-1)+ Dendritic Cell Isolation Kit for simultaneous isolation of all blood dendritic cells (PDCs, MDC2s, and CD1c (BDCA-1)+ type-1 myeloid dendritic cells) and HPCs.
Furthermore, the Anti-PTK7 (CCK-4) MicroBead Kit can be used for the isolation of PDCs if the CD304 (BDCA-4/Neuropilin-1) MicroBead Kit is not suitable, e.g., for the isolation of PDCs from tonsils. It can also be used for isolation of HPCs if the CD34 MicroBead Kit is not applicable.
Columns
For positive selection: MS, LS, or autoMACS® Columns.
 
Figure 1
Isolation of PTK7 (CCK-4)+ cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated.
A) Before magnetic separation
Identification of PDCs and HPCs
Identification of MDC2s
Discrimination between PTK7-expressing cell types
B) Enriched PTK7 (CCK-4)+ cells
Identification of PDCs and HPCs
Identification of MDC2s
Discrimination between PTK7-expressing cell types
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Details
Products
Anti-PTK7 (CCK-4) MicroBead Kit, human
- for 2×109 total cells
Download datasheet
130-091-367
Qty.:
 

Related products
CD303 (BDCA-2) Antibodies
CD141 (BDCA-3) Antibodies
CD34 Antibodies
Anti-PTK7 (CCK-4) Antibodies
CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, human
CD304 (BDCA-4/Neuropilin-1) MicroBead Kit
CD34 MicroBead Kit
References
1. Mossie et al. (1995) Oncogene 11: 2179–2184.
2. Park et al. (1996) J. Biochem. 119: 235–239.
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