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NH Expansion Medium
Description
To better understand the processes that mediate mesenchymal stromal cell (MSC) differentiation into functional nonhematopoietic (NH) cell types, and to identify the factors involved, the establishment of efficient and reproducible procedures for MSC isolation and cultivation is requisite. MACS® NH Expansion Medium efficiently expands cultures of MSCs in vitro while maintaining the cells’ differentiation potential. The medium is supplied in a ready-to-use format and is manufactured under strictly controlled conditions using ingredients of the highest quality. MACS® NH Expansion Medium offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs.
Applications
As MSCs appear at relatively low frequency in human bone marrow (BM) samples and other tissues, their in vitro propagation is often necessary in order to obtain sufficient cell numbers for further experiments, such as in vivo transplantation studies in animals or in vitro differentiation studies including gene expression or protein profiling. The NH Expansion Medium is an optimized and standardized medium for the reproducible and reliable expansion of MSCs from human BM samples or other tissue sources. For small-scale expansions, use the NH CFU-F Medium.
Further information
Preparation of the stromal vascular fraction (SVF) from human lipoaspirate
[PDF; 116,9 KB]
 
Figure 1
Expanded MSCs from BM using the NH Expansion Medium.
Figure 2
MACS® NH Expansion Medium.
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Products
NH Expansion Medium, human
Formulation
Components
- 500 mL
Download data sheet
130-091-680
Qty:
 

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