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MACS® Media MACS® Media
  CHO medium
  MACS® HSC-CFU Media
  MACS® Nonhematopoietic (NH) Stem Cell Media
  MACS® Mo-DC Media
  MACS® Neuro Media
  MACS® Basic Media
MACS® Cytokines MACS® Cytokines
MACS® Cell Stimulation Reagents MACS® Cell Stimulation Reagents
Antigen targeting Antigen targeting
Culture bags Culture bags
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HSC-CFU Media, human
Overview
MACS® HSC-CFU Media have been developed to assess the differentiation potential of human hematopoietic stem and progenitor cells as Colony Forming Units (CFU). They are ideally suited for use with
  • unseparated blood3, bone marrow4, or fetal liver cells
  • sorted cell populations1,4
  • or ES and iPS cell-derived hematopoietic precursors2
Details
MACS® HSC-CFU Media are standardized semi-solid media based on methylcellulose in IMDM, supplemented with FBS, BSA, and different growth factors. MACS® HSC-CFU media are designed to maximize growth and differentiation of progenitor cells and allow the clonal progeny of a single cell to grow in a distinct cluster or colony. They are produced under tightly controlled manufacturing conditions and use highly qualified raw materials to provide a consistent and optimally performing colony assay.

MACS® HSC-CFU media are available in different formats. For details on media composition and supported colony types please see figure 2.
Certificate of Analysis
To receive a CoA (PDF) for one of these products, please click here. In order to open a PDF it is necessary to have Acrobat Reader installed on your computer. You can download this program here, free of charge.
Further information
HSC-CFU media poster Watts 2008
[PDF; 802,5 KB]
 
Figure 1
MACS® HSC-CFU Media.
Figure 2
Formulation of HSC-CFU media.
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Details
Products
HSC-CFU complete with Epo, human
Formulation
Components
- 100 mL
Download datasheet
130-091-280
Qty.:
 

Components
- 24×3 mL
Download datasheet
130-091-278
Qty.:
 

HSC-CFU complete w/o Epo, human
Formulation
Components
- 100 mL
Download datasheet
130-091-277
Qty.:
 

Components
- 24×3 mL
Download datasheet
130-091-276
Qty.:
 

HSC-CFU lite with Epo, human
Formulation
Components
- 100 mL
Download datasheet
130-091-281
Qty.:
 

Components
- 24×3 mL
Download datasheet
130-091-282
Qty.:
 

HSC-CFU basic, human
Formulation
Components
- 80 mL
Download datasheet
130-091-275
Qty.:
 

Related products
CD34 MicroBead Kit
CD133 MicroBead Kit
CD117 MicroBead Kit
Lineage Cell Depletion Kit
References
1. Del Fante et al. (2005) Stem Cells Dev. 14: 734–739.
2. Chang et al. (2006) Blood 108: 1515–1523.
3. Biedermann et al. (2007) Leuk. Res. 31: 211–220.
4. Watts et al. (2008) Cytotherapy 10 (suppl. 1): poster no. 150.
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