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| Description |
| The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC class II antigen. Additionally, CD4 is expressed on cells transfected with pMACS 4.1 or pMACS 4-IRES.II using the MACSelect™ 4 - Transfected Cell Selection Kit. |
| Applications |
| CD4-FITC is used to identify and enumerate of CD4+ cells. It is also used to evaluate the number of cells transfected with pMACS 4.1 or pMACS 4-IRES.II before magnetic labeling and enrichment. Transfected cells are stained with CD4-FITC and analyzed by flow cytometry or fluorescence microscopy to determine the transfection efficiency. |
| Clone | Isotype |
| M-T466 | Mouse IgG1 |
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| Figure 1 |
| CHO cells were transfected with pMACS 4-IRES.II. A sample of the transfected cells was fixed and stained with CD4-FITC to evaluate transfection efficiency. Cell nuclei were counterstained with Toto3 (Applied Biosystems). |
| Transfected cells before enrichment |
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| Details |
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| Products |
| CD4-FITC, human |
- for 100 tests (1)
Download datasheet
130-080-501
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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