CD4-FITC Antibody
Description The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC class II antigen. Additionally, CD4 is expressed on cells transfected with pMACS 4.1 or pMACS 4-IRES.II using the MACSelectâ„¢ 4 - Transfected Cell Selection Kit. Applications CD4-FITC is used to identify and enumerate of CD4+ cells. It is also used to evaluate the number of cells transfected with pMACS 4.1 or pMACS 4-IRES.II before magnetic labeling and enrichment. Transfected cells are stained with CD4-FITC and analyzed by flow cytometry or fluorescence microscopy to determine the transfection efficiency. Clone Isotype M-T466 Mouse IgG1		Figure 1 CHO cells were transfected with pMACS 4-IRES.II. A sample of the transfected cells was fixed and stained with CD4-FITC to evaluate transfection efficiency. Cell nuclei were counterstained with Toto3 (Invitrogen). Transfected cells before enrichment

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