MACSelect™ LNGFR System
The MACSelect™ LNGFR System uses the truncated human low-affinity nerve growth factor receptor (LNGFR) molecule as a marker to select transfected cells. It can be used in virtually all LNGFR-negative cell lines and primary cells. The LNGFR molecule is expressed in the central and peripheral nervous system, on bone marrow fibroblasts, follicular dendritic cells, and some mesenchymal cells. pMACS LNGFR-IRES allows the bicistronic expression of the gene-of-interest and the truncated LNGFR molecule: with the IRES element (internal ribosomal entry site), the gene-of-interest is translated from the same mRNA as the selection marker LNGFR. The pMACS LNGFR vector encoding the truncated LNGFR surface marker is used for cotransfection in combination with a gene-of-interest expression vector. The MACSelect LNGFR Transfected Cell Selection Kit provides the vectors, MACSelect MicroBeads, and fluorochrome-conjugated antibodies, including control vectors and antibodies required to set up and establish the MACSelect System. All kit components are also available separately. Columns For positive selection: MS, LS, or autoMACS® Columns. Disclaimer The CMV promoter is covered under U.S. patents 5,168,062 and 5,385,839 and its use is permitted for research use only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242. The EMCV IRES sequence element is covered under U.S. patent 4,937,190 and its use is permitted for research purposes only. Any other use of the EMCV IRES element requires a licence from Wisconsin Alumni Research Foundation. Further information DNA sequence pMACS LNGFR (RTF Document, MAC) [RichTextFile (RTF); 8,1 KB] DNA sequence pMACS LNGFR (Text Document, PC) [Raw Text File; 5,3 KB] DNA sequence pMACS LNGFR IRES (RTF Document, MAC) [RichTextFile (RTF); 8,1 KB] DNA sequence pMACS LNGFR IRES (Text Document, PC) [Raw Text File; 5,3 KB] vector map pMACS 14.1 (Single Cutting Restriction Sites) [PDF; 20,3 KB] vector map pMACS 14.1 (Twice Cutting Restriction Sites) [PDF; 22,3 KB] vector map pMACS LNGFR [PDF; 37,6 KB] vector map pMACS LNGFR-IRES [PDF; 57,6 KB] pMACS LNGFR-IRES location of features [PDF; 54,1 KB]		Figure 1 The pMACS LNGFR-IRES vector. Figure 2 The pMACS LNGFR vector. Figure 3 MACSelect transfected cell enrichment

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