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The MACSelect™ Kk System uses the truncated mouse MHC class I molecule H-2Kk as a selection marker. H-2Kk expression is restricted to some rarely used mouse strains, e.g. AKR/J or CBA/J. MACSelect Kk can be used in virtually any mammalian cell line and in primary cells. The H-2Kk marker should not be used when MHC class I expression is down-regulated either directly or via reduced expression of β2 microglobulin, e.g. in some tumor cell lines such as adenocarcinoma cell lines.
The pMACS Kk.II vector encodes the truncated H-2Kk surface marker and can be used for cloning the gene-of-interest. Additionally, it can be used for cotransfection in combination with a gene-of-interest expression vector.
The MACSelect Kk Transfected Cell Selection Kit provides the vectors, MACSelect MicroBeads, and fluorochrome-conjugated antibodies, including control vectors and antibodies required to set up and establish the MACSelect System. All kit components are also available separately. |
| Further information |
vector map pMACS Kk.II (Single Cutting Restriction Sites)
[PDF; 63,3 KB]
DNA sequence pMACSKkII (RTF Document, MAC)
[RichTextFile (RTF); 7,9 KB]
vector map pMACS 14.1 (Single Cutting Restriction Sites)
[PDF; 20,3 KB]
vector map pMACS 14.1 (Twice Cutting Restriction Sites)
[PDF; 22,3 KB]
DNA sequence pMACS_Kk_HA(C) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB]
DNA sequence pMACS_Kk_HA(N) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB]
DNA sequence pMACS_Kk_c-myc(C) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB]
DNA sequence pMACS_Kk_c-myc(N) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB]
DNA sequence pMACS_Kk_His(C) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB]
DNA sequence pMACS_Kk_His(N) (RTF Document, MAC)
[RichTextFile (RTF); 5,5 KB] |
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| Figure 1 |
| The pMACS Kk.II vector. |
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| Figure 2 |
| A, B, and C represent various cell numbers of CHO cells before and after enrichment for the enrichment of CHO cells. Cells were cotransfected with pMACS Kk.II and pMACS 14.1 encoding CD14 as the gene-of-interest. |
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| Figure 3 |
| MACSelect transfected cell enrichment |
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| Favorites / Prices |
Request prices or add products to your favorite list.
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| Details |
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| Products |
| MACSelect Kk – Transfected Cell Selection Kit |
- for 25 separations
Components
- 25 μg pMACS Kk.II
- 25 μg pMACS 14.1
- 0.25 mL MACSelect Control FITC Antibody
- 0.25 mL Anti-H-2Kk-FITC, mouse
- 0.25 mL CD14-FITC, human
- 2 mL MACSelect Kk MicroBeads
Download datasheet
130-091-986
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| pMACS Kk.II |
Components
- 25 μg plasmid
Download datasheet
130-091-889
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| MACSelect Kk MicroBeads |
- for 25 separations
Components
- 2 mL MACSelect Kk MicroBeads
Download datasheet
130-070-201
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| References |
| 1. Petry et al. (1999) Int. Immunol. 11: 753-763. |
| 2. Tetsu and McCormick (1999) Nature 398: 422-426. |
| 3. Porter et al. (2002) J. Immunol. 168: 4936-4945. |
| 4. Finotto et al. (2001) J. Exp. Med. 193: 1247-1260. |
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