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MACSelectâ„¢ 4 System
The MACSelectâ„¢ 4 System uses the truncated human CD4 molecule as a marker to select transfected cells. It can be used in virtually all CD4-negative cell lines and primary cells.*

pMACS 4-IRES.II enables the bicistronic expression of the gene-of-interest and the truncated CD4 molecule: with the IRES element (internal ribosomal entry site), the gene-of-interest is translated from the same mRNA as the selection marker CD4.
The pMACS 4.1 vector encoding the truncated CD4 surface marker is used for co-transfection in combination with a gene-of-interest expression vector.

The MACSelect 4 Transfected Cell Selection Kit provides the vectors, MACSelect MicroBeads, and fluorochrome-conjugated antibodies, including control vectors and antibodies required to set up and establish the MACSelect System. All kit components are also available separately.


* The CD4 molecule is sensitive to trypsin treatment and should, therefore, not be used in cell lines which need to be harvested with trypsin.
Columns
For positive selection: MS, LS, or autoMACSâ„¢ Columns.
Disclaimer
The CMV promoter is covered under U.S. patents 5,168,062 and 5,385,839 and its use is permitted for research purposes only. Any other use of the CMV promoter requires a licence from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.
Further information
Plasmid transfection protocols
[PDF; 139,8 KB]
DNA sequence pMACS 4-IRES.II (Text Document, PC)
[Raw Text File; 5,7 KB]
DNA sequence pMACS 4-IRES.II (RTF Document, MAC)
[RichTextFile (RTF); 10,5 KB]
vector map pMACS 14.1 (Single Cutting Restriction Sites)
[PDF; 20,3 KB]
vector map pMACS 14.1 (Twice Cutting Restriction Sites)
[PDF; 22,3 KB]
vector map pMACS 4.1 (Single Cutting Restriction Sites)
[PDF; 22 KB]
vector map pMACS 4.1 (Twice Cutting Restriction Sites)
[PDF; 23,7 KB]
DNA sequence pMACS 4.1 (Text Document, PC)
[Raw Text File; 4,4 KB]
 
Figure 1
The pMACS 4-IRES.II vector.
* The BsmB I site is compatible with Hind III digested fragments.
Figure 2
The pMACS 4.1 vector.
Figure 3
Transfection of CHO cells with pMACS 4-IRES.II, magnetic labeling with MACSelect 4 MicroBeads and separation. FITC staining of transfected cells (CD4-FITC/MACSelect Control FITC), blue nuclear counterstain (Toto 3, Molecular Probes).
A: Less than 4% transfected cells after transfection
B: More than 90% transfected cells after MACSelect enrichment
Figure 4
MACSelect transfected cell enrichment
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Products
MACSelect 4 - Transfected Cell Selection Kit
for 25 separations
Components:
- 25 μg pMACS 4-IRES.II
- 25 μg pMACS 4.1
- 25 μg pMACS 14.1
- 0.25 mL MACSelect Control FITC Antibody
- 0.25 mL CD4-FITC, human
- 0.25 mL CD14-FITC, human
- 2 mL MACSelect 4 MicroBeads
Download data sheet
130-091-988
Qty:
 

pMACS 4.1
Components:
- 25 μg plasmid
Download data sheet
130-091-886
Qty:
 

pMACS 4-IRES.II
Components:
- 25 μg plasmid
Download data sheet
130-091-888
Qty:
 

MACSelect 4 MicroBeads
for 25 separations
Components:
- 2 mL MACSelect 4 MicroBeads
Download data sheet
130-070-101
Qty:
 

Related products
CD4-FITC, human (#130-080-501)
CD14-FITC, human (#130-080-701)
MACSelect Control FITC Antibody (#130-090-326)
Dead Cell Removal Kit (#130-090-101)
MACS References
1. David et al. (2005) Stem Cells 23: 477-482.[6930]
2. Philipps et al. (2004) BioTechniques 36: 80-83.[3783]
3. Szentirmay et al. (2003) J. Biol. Chem. 278: 37231-37240.[3086]
4. Kube et al. (1999) J. Virol. 73: 1630-1636.[896]
5. von Knethen et al. (1999) Macrophages. Mol. Biol. Cell 10: 361-372.[891]
6. Gaines et al. (1999) BioTechniques 26: 683-688.[888]
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