|
|
 |
 - Effectively remove myelin from neural cell suspensions
- Improve the performance of downstream applications
- No more need for determination of cell/debris ratio or cell numbers
|
| Overview |
| Myelin Removal Beads II have been developed for the specific removal of myelin debris from single-cell suspensions. Myelin Removal Beads II work without determination of cell number or myelin debris/cell ratio. |
| Details |
Background information
When myelin-containing neural tissue is dissociated, large quantities of myelin debris are generated, which considerably impair antibody binding.1,2
Depletion of myelin debris from single-cell suspensions leads to improved immunostaining and cell separation results. |
| |
| Figure 1 |
| Mouse brains (P28) were dissociated using the Neural Tissue Dissociation Kit (P). Flow cytometric analysis shows that the resulting single-cell suspension contains large amounts of myelin membrane fragments and other cellular debris. Only 3% of the signals represent cells (A). Myelin Removal Beads II used with an LS Column and a MidiMACS Separator effectively remove myelin debris (B). |
| A |
 |
| B |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| References |
| 1. Pfenninger et al. (2007) Cancer Res. 67: 5727–5736. |
| 2. Tham et al. (2003) Int. J. Dev. Neurosci. 21: 431–443. |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
