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Sample dissociation Sample dissociation
  gentleMACS Dissociator
  gentleMACS Octo Dissociator
  gentleMACS Tubes
  Tissue Dissociation Kits
Sample clearing Sample clearing
Sample mixing Sample mixing
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Spleen Dissociation Kit, mouse
Description
The Spleen Dissociation Kit has been developed for the gentle and rapid generation of single-cell suspensions from mouse spleen. It is optimized for high yield of leukocytes, especially dendritic cells, while preserving cell surface epitopes. The kit is ideally suited for the time-saving and reproducible preparation of single-cell suspensions in combination with the gentleMACS™ Dissociator and the gentleMACS Octo Dissociator.
Applications
The Spleen Dissociation Kit is recommended for the generation of single-cell suspensions from mouse spleen prior to the isolation of dendritic cells, for example, when using CD11c MicroBeads, Anti-mPDCA-1 MicroBeads, or Pan DC MicroBeads.
 
Figure 1
Mouse spleen single-cell suspensions were stained with CD11c-APC and Anti-mPDCA-1-FITC and analyzed using the MACSQuant® Analyzer. Viable leukocytes are shown.
Figure 2
Mouse spleen single-cell suspensions were stained with CD8a-FITC and CD4-PE and analyzed using the MACSQuant® Analyzer. Viable leukocytes are shown.
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Products
Spleen Dissociation Kit, mouse
- for 50 preparations
Download datasheet
130-095-926
Qty.:
 

Related products
CD11c MicroBeads
Anti-mPDCA-1 MicroBeads
Pan DC MicroBeads
gentleMACS Dissociator
gentleMACS Octo Dissociator (#130-095-937)
gentleMACS™ C Tubes
Pre-Separation Filters, 30 µm
MACSmix™ Tube Rotator
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