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| Whole Blood CD138 MicroBeads |
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| Overview |
| Whole Blood CD138 MicroBeads were developed for rapid positive selection of CD138+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation and erythrocyte lysis. |
| Details |
Background information
The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. In peripheral blood of healthy donors approximately 50% of plasma cells express this antigen. In contrast, CD138 is not expressed by naive B cells, germinal center B cells, memory B cells, T cells, and monocytes.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD138 MicroBeads and subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit. Whole Blood MicroBeads in combination with the autoMACS Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.
Downstream applications
The purified CD138+ cells are well suited for flow cytometric, functional, or molecular analysis. |
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| Figure 1 |
| CD138+ cells were separated from whole blood using Whole Blood CD138 MicroBeads and the autoMACS® Pro Separator with program Posseldwb. Cells were fluorescently stained with CD138-PE and CD19-APC. |
| Isolated CD138+ cells |
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| Details |
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| Products |
| Whole Blood CD138 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-093-062
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