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| Whole Blood CD15 MicroBeads |
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| Overview |
| Whole Blood CD15 MicroBeads have been developed for rapid positive selection of CD15+ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis. |
| Details |
Background information
Whole Blood CD15 MicroBeads recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine (3-FAL), also designated Lewis X. The CD15 antigen is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
Downstream applications
Whole Blood MicroBeads allow the fast isolation of CD15+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD15+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2,3. |
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| Figure 1 |
| CD15+ cells were separated from whole blood using Whole Blood CD15 MicroBeads and the autoMACS® Separator with program Posseld2. Cells were fluorescently stained with CD15-FITC and CD45-PE. |
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| Favorites / Prices |
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| Details |
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| Products |
| Whole Blood CD15 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-091-058
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| References |
| 1. Köhl et al. (2003) Leukemia 17: 232–236. |
| 2. Adams et al. (2004) MACS&more 8/2: 16–17. |
| 3. Meyer et al. (2006) Blood 109: 374–382. |
| 4. Willasch et al. (2010) Bone Marrow Transplantation 45: 181–189. |
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