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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  Anti-Fluorochrome MicroBeads
  Anti-Biotin MicroBeads
  Streptavidin MicroBeads
  Monoclonal Anti-Ig MicroBeads
  Polyclonal Anti-Ig MicroBeads
  MACSiBead™ Particles
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Anti-Biotin MACSiBead™ Particles
Overview
Anti-Biotin MACSiBead Particles have been developed for the depletion of cells labeled with biotinylated primary antibodies. They are, for example, suitable for depletion of non-target cells from lymphoid tissue. Anti-Biotin MACSiBead Particles can also be used for two-step magnetic separation by an initial depletion with Anti-Biotin MACSiBead Particles followed by positive selection with MACS MicroBeads.
Details
Background information
Anti-Biotin MACSiBead™ Particles consist of MACSiBead Particles covalently coupled to anti-biotin antibodies. MACSiBead Particles are superparamagnetic beads of 3.5 μm diameter. They are not biodegradable. The monodisperse, hydrophilic MACSiBead Particles show no autofluorescence. Magnetic separation is performed using the MACSiMAG™ Separator.

Note: MACSiBead Particles are not suitable for separations using MACS Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, or autoMACS® Separators.
 
Figure 1
Depletion of T cells from human PBMCs using CD3-Biotin and Anti-Biotin MACSiBead Particles. Cells are fluorescently stained with CD3-APC.
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Details
Products
Anti-Biotin MACSiBead Particles
- for 5×108 total cells
Download datasheet
130-091-147
Qty.:
 

Related products
MACSmix Tube Rotator (#130-090-753)
MACSiMAG Separator (#130-092-168)
Anti-Biotin antibodies
One-Step Antibody Biotinylation Kit (#130-093-385)
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