| Description |
| Anti-Biotin MACSiBead™ Particles consist of MACSiBead Particles covalently coupled to anti-biotin antibodies. MACSiBead Particles are superparamagnetic beads of 3.5 μm diameter. They are not biodegradable. The monodisperse, hydrophilic MACSiBead Particles show no autofluorescence. |
| Applications |
| Anti-Biotin MACSiBead Particles have been developed for the depletion of cells labeled with biotinylated primary antibodies. They are, for example, suitable for depletion of non-target cells from lymphoid tissue. Anti-Biotin MACSiBead Particles can also be used for two-step magnetic separation by an initial depletion with Anti-Biotin MACSiBead Particles followed by positive selection with MACS® MicroBeads. |
| Required equipment |
Magnetic separation is performed using the MACSiMAGâ„¢ Separator.
Note: MACSiBead Particles are not suitable for separations using MACS Columns and MiniMACSâ„¢, MidiMACSâ„¢, VarioMACSâ„¢, SuperMACSâ„¢, or autoMACSâ„¢ Separators. |
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| Figure 1 |
| Depletion of T cells from human PBMCs using CD3-Biotin and Anti-Biotin MACSiBead Particles. Cells are fluorescently stained with CD3-APC. |
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