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| Anti-Rabbit IgG MicroBeads |
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| Overview |
| Anti-Rabbit IgG MicroBeads, formerly known as Goat Anti-Rabbit IgG MicroBeads, can be used for magnetic labeling and subsequent separation of cells or other target materials labeled with rabbit antiserum. Anti-Rabbit IgG MicroBeads react with the heavy chains of all rabbit IgG antibodies. These MicroBeads are conjugated with polyclonal goat F(ab')2 fragments which have been affinity-adsorbed to remove cross-reactivity to human immunoglobulins. |
| Details |
Downstream applications
The versatility of Anti-Rabbit IgG MicroBeads was shown by the isolation of murine CD34+ progenitor cells1 and the isolation of TGFα+ cells from human bone marrow2. Anti-Rabbit MicroBeads have also been used for the isolation of Cryptosporidium parvum oocysts from spiked samples3 and for the enrichment of spermatogonia from Djungarian hamster, mouse, and marmoset monkey testes4. |
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| Figure 1 |
| Separation of mouse spleen cells using a FITC-conjugated rabbit anti-mouse IgG antibody, Anti-Rabbit IgG MicroBeads, and a MiniMACS™ Separator with an MS Column. |
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| Details |
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| References |
| 1. Cheng et al. (1996) Blood 87: 479–490. |
| 2. Walz et al. (1995) Blood 9: 2385–2392. |
| 3. Deng et al. (2000) J. Microbiol. Meth. 40: 11–17. |
| 4. von Schönfeldt et al. (1999) Biol. Reprod. 61: 582–589. |
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