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Goat Anti-Rat IgG MicroBeads
Description
Goat Anti-Rat IgG MicroBeads can be used in combination with any primary rat IgG antibody for the magnetic labeling and separation of cells. Goat Anti-Rat IgG MicroBeads recognize the heavy and light chains of all rat IgG isotypes. The goat F(ab')2 fragments have been affinity adsorbed to remove any cross-reactivity to mouse immunoglobulins.
Applications
Goat Anti-Rat IgG MicroBeads have been used for the isolation of murine leukocyte subsets from brain tissue1, for the separation of murine T cell subsets from PBMCs2, for the isolation of murine dermal macrophages3 as well as for the isolation of intraepithelial CD8β+ lymphocytes from murine intestine4. They have also been used for the separation of CD105- and CD106-expressing endothelial cells from murine aortic tissue.5
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Separation of mouse spleen cells using rat anti-mouse CD45R antibody (isotype: IgG2a), Goat Anti-Rat IgG MicroBeads, and a MiniMACS™ Separator with an MS Column.
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Goat Anti-Rat IgG MicroBeads
for 5Ă—108 total cells
Download data sheet
130-048-502
Qty:
 

for 109 total cells
Download data sheet
130-048-501
Qty:
 

MACS References
1. SchlĂĽter et al. (1996) Am. J. Pathol. 150: 1021-1035.[290]
2. Schön et al. (1997) Nat. Med. 3: 183-188.[385]
3. Sato et al. (1998) J. Immunol. 161: 6835-6844.[853]
4. Lepage et al. (1998) J. Immunol. 168: 4902-4908.[815]
5. Ploplis et al. (2004) J. Biol. Chem. 279: 6143-6151.[3971]
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