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| Overview |
| Anti-Rat IgG MicroBeads, formerly known as Goat Anti-Rat IgG MicroBeads, can be used in combination with any primary rat IgG antibody for the magnetic labeling and separation of cells. Anti-Rat IgG MicroBeads recognize the heavy and light chains of all rat IgG isotypes. The goat F(ab')2 fragments have been affinity adsorbed to remove any cross-reactivity to mouse immunoglobulins. |
| Details |
Downstream applications
Anti-Rat IgG MicroBeads have been used for the isolation of murine leukocyte subsets from brain tissue1, for the separation of murine T cell subsets from PBMCs2, for the isolation of murine dermal macrophages3 as well as for the isolation of intraepithelial CD8β+ lymphocytes from murine intestine4. They have also been used for the separation of CD105- and CD106-expressing endothelial cells from murine aortic tissue.5 |
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| Figure 1 |
| Separation of mouse spleen cells using rat anti-mouse CD45R antibody (isotype: IgG2a), Anti-Rat IgG MicroBeads, and a MiniMACS™ Separator with an MS Column. |
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| References |
| 1. Schlüter et al. (1996) Am. J. Pathol. 150: 1021–1035. |
| 2. Schön et al. (1997) Nat. Med. 3: 183–188. |
| 3. Sato et al. (1998) J. Immunol. 161: 6835–6844. |
| 4. Lepage et al. (1998) J. Immunol. 168: 4902–4908. |
| 5. Ploplis et al. (2004) J. Biol. Chem. 279: 6143–6151. |
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