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| Anti-Mouse IgG MicroBeads |
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| Overview |
| Anti-Mouse IgG MicroBeads, formerly known as Goat Anti-Mouse IgG MicroBeads, recognize the heavy and light chains of all mouse IgG antibodies (IgG1, IgG2a, IgG2b, and IgG3). They also react with light chains of other mouse immunoglobulin classes like IgM, IgA, or IgE. Goat Anti-Mouse IgG MicroBeads are conjugated with goat F(ab')2 fragments which have been affinity-adsorbed to remove cross-reactivity with human immunoglobulins. |
| Details |
Downstream applications
Anti-Mouse IgG MicroBeads have been used for numerous different cell separations, including tumor cells from patients blood1,2, fetal trophoblasts from maternal blood3, human T cell subsets4, and human NK cells5 from PBMCs as well as the isolation of chicken osteocytes from chicken calvariae and tibiotarsi. Positive selection of trout thrombocytes6, a subpopulation of rat macrophages7, and porcine hematopoietic stem cells8 with Anti-Mouse IgG MicroBeads has been reported, as well as the depletion of B cells from Xenopus laevis spleen9. |
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| Figure 1 |
| Chicken osteocytes isolated from chicken calvariae and tibiotarsi using mAb 7.3 and Anti-Mouse IgG MicroBeads. (Courtesy of Dr. Suswillo, London, UK.) |
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| References |
| 1. Wong et al. (1995) Br. J. Surgery 82: 1333–1337. |
| 2. Benez et al. (1999) J. Clin. Lab. Anal. 13: 229–233. |
| 3. van Wijk et al. (1996) Am. J. Obstet. Gynecol. 174: 871–876. |
| 4. Caux et al. (1996) J. Exp. Med. 184: 695–706. |
| 5. Geiselhart et al. (1997) Natural Immunity 15: 227–233. |
| 6. Hill et al. (1999) Biochim. Biophys. Acta 1437: 63–70. |
| 7. Massimino et al. (1997) Biochem. Biophys. Res. Commun. 235: 754–759. |
| 8. Layton et al. (2007) Exp. Hematol. 35: 171–178. |
| 9. Horton et al. (2000) Eur. J. Immunol. 30: 604–613. |
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