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 - Convenient: Ready-to-use lyophilized PCR mixes
- Informative: highly polymorphic STR markers, extensively validated for related individuals
- Reliable: accurate quantification and sensitive detection of donor and recipient cells
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| Overview |
| The ChimerXplain and ChimerXact Kits have been developed for reliable chimerism analysis after allogeneic transplantation. |
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Background information
The ChimerXplain Kit allows co-amplification of ten highly polymorphic STR loci, D2S1360, D7S1517, D8S1132, D9S1118, D10S2325, D11S554, D12S391, MYCL1, P450CYP19, and SE-33, which have been extensively validated for related individuals (>500 pairs). This panel provides at least two informative STR markers which meet the stringent criteria of eligibility defined by the EuroChimerism consortium in >99% of all recipient/donor constellations.
After the initial identification of suited STR markers, further chimerism analysis can be carried out using the ChimerXact Kits 1- 10 for the amplification of individual STR markers. These singleplex assays allow not only the accurate and reproducible quantification of cells of donor and recipient origin but also the sensitive detection of residual recipient cells at a percentage of below 3%.
For each STR comprised in the ChimerXplain Kit, a corresponding ChimerXact Kit is available, allowing singleplex PCR reactions. |
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| Figure 1 |
| Overview on size range of amplified PCR products. Dark purple: labeled with FL; purple: labeled with Joe; light purple with purple font: labeled with TMR. |
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