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Rat Anti-Mouse IgM MicroBeads
Description
Rat Anti-Mouse IgM MicroBeads are suitable for indirect magnetic labeling and subsequent separation of cells where the primary antibody is of the mouse IgM isotype.
Applications
Rat Anti-Mouse IgM MicroBeads have been used for the isolation of lymphocyte subsets1, for the isolation of murine primordial germ cells2 as well as for the isolation of follicular dendritic cells from human tonsils3. Rat Anti-Mouse IgM MicroBeads have also been used for direct positive selection or depletion of mouse B cells expressing IgM on the surface.4,5
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.
Further information
Positive Selection of Antibody Expressing Hybridoma Cells
[PDF; 74,8 KB]
 
Figure 1
Separation of CD16+ cells from human peripheral blood leukocytes using a mouse anti-human CD16 antibody (isotype IgM), Rat Anti-Mouse IgM MicroBeads, and an MS Column.
Before separation
Positive fraction
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Rat Anti-Mouse IgM MicroBeads
- for 5Ă—108 total cells
Download data sheet
130-047-302
Qty:
 

- for 109 total cells
Download data sheet
130-047-301
Qty:
 

MACS References
1. Diacovo et al. (1996) J. Exp. Med. 183: 1193-1203.[268]
2. Pesce and De Felici (1995) Dev. Biol. 170: 722-725.[309]
3. Butch et al. (1999) Blood 94: 216-224.[588]
4. Pelanda et al. (1996) Immunity 5: 229-239.[311]
5. Underhill et al. (2002) Blood: 2905-2912.[4302]
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