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Rat Anti-Mouse IgG2a+b MicroBeads
Description
Rat Anti-Mouse IgG2a+b MicroBeads can be used for magnetic labeling and subsequent separation of cells where the primary antibody is of the mouse IgG2a or IgG2b isotype. Furthermore, Rat Anti-Mouse IgG2a+b MicroBeads can be used for direct positive selection or depletion of mouse B cells expressing IgG2a or IgG2b on their surface.
Applications
Rat Anti-Mouse IgG2a+b MicroBeads have been utilized for the separation of human T cell subsets1 as well as for the enrichment of fetal erythrocytes2, epithelial tumor cells3, or bovine leukocytes4.
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.
Further information
Positive Selection of Antibody Expressing Hybridoma Cells
[PDF; 74,8 KB]
 
Figure 1
Separation of monocytes from human PBMCs using a mouse anti-human CD14 antibody (isotype: IgG2a), Rat Anti-Mouse IgG2a+b MicroBeads, and a MiniMACS™ Separator with an MS Column.
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Rat Anti-Mouse IgG2a+b MicroBeads
- for 5Ă—108 total cells
Download data sheet
130-047-202
Qty:
 

- for 109 total cells
Download data sheet
130-047-201
Qty:
 

References
1. Jung et al. (1993) J. Immunol. Meth. 159: 197-207.[70]
2. Zheng et al. (1993) J. Med. Genet. 30: 1051-1056.[137]
3. Griwatz et al. (1995) J. Immunol. Meth. 183: 251-265.[145]
4. Brooke et al. (1998) Eur. J. Immunol. 28: 1-11.[433]
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