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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  Anti-Fluorochrome MicroBeads
  Anti-Biotin MicroBeads
  Streptavidin MicroBeads
  Monoclonal Anti-Ig MicroBeads
  Polyclonal Anti-Ig MicroBeads
  MACSiBead™ Particles
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Anti-Mouse IgG2a+b MicroBeads
Overview
Anti-Mouse IgG2a+b MicroBeads can be used for magnetic labeling and subsequent separation of cells where the primary antibody is of the mouse IgG2a or IgG2b isotype. They can also be used for direct positive selection or depletion of mouse B cells expressing IgG2a or IgG2b on their surface.
Details
Applications
Anti-Mouse IgG2a+b MicroBeads have been utilized for the separation of human T cell subsets1 as well as for the enrichment of fetal erythrocytes2, epithelial tumor cells3, or bovine leukocytes4.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
Further information
Positive Selection of Antibody Expressing Hybridoma Cells
[PDF; 76,7 KB]
 
Figure 1
Separation of monocytes from human PBMCs using a mouse anti-human CD14 antibody (isotype: IgG2a), Anti-Mouse IgG2a+b MicroBeads, and a MiniMACS™ Separator with an MS Column.
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Details
Products
Anti-Mouse IgG2a+b MicroBeads
- for 5×108 total cells
Download datasheet
130-047-202
Qty.:
 

- for 109 total cells
Download datasheet
130-047-201
Qty.:
 

Related products
CD19 Antibodies
Anti-IgG2ab Antibodies
References
1. Jung et al. (1993) J. Immunol. Meth. 159: 197–207.
2. Zheng et al. (1993) J. Med. Genet. 30: 1051–1056.
3. Griwatz et al. (1995) J. Immunol. Meth. 183: 251–265.
4. Brooke et al. (1998) Eur. J. Immunol. 28: 1–11.
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