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| Anti-Mouse IgG1 MicroBeads |
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| Overview |
| Anti-Mouse IgG1 MicroBeads can be used for magnetic labeling and subsequent separation of cells, which are labeled with a primary mouse IgG1 antibody. They can also be used for positive selection or depletion of mouse B cells expressing IgG1 on the cell surface. |
| Details |
Downstream applications
Anti-Mouse IgG1 MicroBeads have been used for the isolation of T cells and T cell subpopulations1, B cell subsets2, rat type I alveolar epithelial cells3, and for the isolation of rare cells, such as subsets of kidney cells4, ovarian carcinoma cells5, or myeloma cells6. The isolation of keratinocytes by depletion of non-keratinocytes has also been performed with Anti-Mouse IgG1 MicroBeads.7 |
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| Figure 1 |
| Human kidney cell subsets isolated by using an anti-Tamm-Horsefall glycoprotein antibody for distal tubulus cells and a CD13 antibody for proximal tubulus cells in combination with Anti-Mouse IgG1 MicroBeads. (Courtesy of Dr. Baer, Frankfurt a.M., Germany.) |
| Kidney cells before MACS® Separation |
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| Purified distal tubulus cells |
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| Purified proximal tubulus cells |
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| Details |
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| References |
| 1. Jung et al. (1993) J. Immunol. Meth. 159: 197–207. |
| 2. Näsman et al. (1996) Blood 87: 2795–2804. |
| 3. Dobbs et al. (1998) Proc. Natl. Acad. Sci. USA 95: 2991–2996. |
| 4. Baer et al. (1997) Kidney Int. 52: 1321–1331. |
| 5. Harbeck et al. (1995) Int. J. Oncol. 6: 1249–1254. |
| 6. Drach et al. (1995) Cancer Res. 55: 3854–3859. |
| 7. Formanek et al. (1998) Eur. Arch. Otorhinolaryngol. 255: 211–215. |
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