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| Description |
| Streptavidin MicroBeads are used for the indirect magnetic labeling and separation of cells, subcellular material, or bacteria labeled with a biotinylated primary antibody or ligand. |
| Applications |
| Streptavidin MicroBeads have been used for the isolation of human allergen-specific B cells1 or follicular dendritic cells2, murine liver sinusoidal endothelial cells3, sheep B cell subpopulations4, and rat retinal ganglion cells5. They have also been used in many other separations such as the isolation of plant protoplasts.6 |
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| Figure 1 |
| Potato protoplasts isolated by MACS® Technology using biotinylated lectins and Streptavidin MicroBeads. (Courtesy of Dr. Dörr, Cologne, Germany.) |
| Positively selected protoplasts |
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| Negative fraction |
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| Figure 2 |
| Follicular dendritic cells isolated by MACS® Technology from human tonsils using Streptavidin MicroBeads. (Courtesy of Dr. J. Schmitz, Hamburg, Germany.) |
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| References |
| 1. Zürcher et al. (1995) Immunol. Lett. 46: 49–57. |
| 2. Schmitz et al. (1993) J. Immunol. Meth. 159: 189–196. |
| 3. Do et al. (1999) J. Biol. Chem. 274: 19587–19592. |
| 4. Gupta et al. (1998) Eur. J. Immunol. 28: 1597–1603. |
| 5. Shoge et al. (1999) Neurosci. Lett. 259: 111–114. |
| 6. Dörr et al. (1994) Biotechnology 12: 511–515. |
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