Anti-Biotin MicroBeads
Description Anti-Biotin MicroBeads have been developed for indirect magnetic labeling and separation of cells or other biological materials that are labeled with a biotinylated primary antibody or ligand, or with a cocktail of biotinylated antibodies. The biotinylated molecule is recognized by a monoclonal anti-biotin antibody coupled to MicroBeads. Anti-Biotin MicroBeads have the advantage of not binding to free biotin, which is often present in culture media. This feature provides the most sensitive labeling and separation of cells. Applications Anti-Biotin MicroBeads are ideal for positive selection, even of cells with low antigen expression, or for depletion. Anti-Biotin MicroBeads have been used for the separation of murine αE-CD25+ and αE-CD25- T cell subsets1 and for the isolation of CD45.2+ cells from the bone marrow of chimeric mice2. They have also been used for the enrichment of human CD94+ NK cells.3 Columns For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.		Figure 1 Separation of CD3+ cells from human PBMCs using a biotinylated CD3 antibody, Anti-Biotin MicroBeads, and an MS Column. PBMCs before separation CD3- cells CD3+ cells