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| Overview |
The Whole Blood Column Kit is suited for the separation of leukocyte subsets directly from anticoagulated human whole blood or bone marrow. The kit contains Whole Blood Columns and Whole Blood Column Elution Buffer for convenient positive selection of cells labeled with Whole Blood MicroBeads.
Whole Blood Columns are designed for use with:
- MidiMACS™ Separator
- QuadroMACS™ Separator
- VarioMACS™ Separator, in combination with an LS Column Adapter
- SuperMACS™ II Separator
Column capacity
Up to 15 mL whole blood |
| Details |
Background information
Whole Blood Columns in combination with Whole Blood MicroBeads allow cell separation directly from whole blood, without density-gradient centrifugation and erythrocyte lysis. Optimal flow rates of whole blood samples during cell separation are achieved by a specially developed two-layer column matrix.
The matrix of Whole Blood Columns consists of ferromagnetic spheres and a small layer of non-magnetic spheres. The matrix is covered with a cell-friendly coating. When placed in the magnetic field of a MACS Separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells that are only minimally labeled with MACS MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.
The columns are individually packed in a sterile way. |
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| Figure 1 |
| Whole Blood Column |
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| Details |
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| Products |
| Whole Blood Column Kit |
Components
- 10 Whole Blood Columns
- 50 mL Whole Blood Column Elution Buffer
Download datasheet
130-093-545
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| Related products |
| MidiMACS Separator (#130-042-302) |
| QuadroMACS Separator (#130-090-976) |
| VarioMACS Separator (#130-090-282) |
| SuperMACS II Separator (#130-044-104) |
| MACS MultiStand (#130-042-303) |
| LS Column Adapter (#130-090-544) |
| Whole Blood CD3 MicroBeads, human (#130-090-874) |
| Whole Blood CD4 MicroBeads, human (#130-090-877) |
| Whole Blood CD8 MicroBeads, human (#130-090-878) |
| Whole Blood CD14 MicroBeads, human (#130-090-879) |
| Whole Blood CD15 MicroBeads, human (#130-091-058) |
| Whole Blood CD19 MicroBeads, human (#130-090-880) |
| Whole Blood CD33 MicroBeads, human (#130-094-773) |
| Whole Blood CD45 MicroBeads, human (#130-090-872) |
| Whole Blood CD56 MicroBeads, human (#130-090-875) |
| Whole Blood CD138 MicroBeads, human (#130-093-062) |
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