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| Overview |
LD Columns are specially designed for stringent depletion of unwanted cells. Even weakly labeled cells are efficiently retained on the column.
LD Columns can be used with the following separators:
- MidiMACS™ Separator
- QuadroMACS™ Separator
- VarioMACS™ Separator, in combination with an LS Column Adapter
- SuperMACS™ II Separator
Column capacity
For lymphoid cells from blood, bone marrow or tissue:
Up to 108 magnetically labeled cells from up to 5×108 total cells.
For the capacity for cells from other tissues, e.g., neural tissues, please refer to the data sheet provided with the particular MACS® Cell Separation Reagent. |
| Details |
Background information
The matrix of LD Columns is composed of ferromagnetic spheres, which are covered with a cell-friendly coating. When placed in the magnetic field of a MACS Separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells which are only minimally labeled with MACS MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.
LD Columns can be used to separate material less than 30 μm in size. The columns are individually packed in a sterile way. |
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| Figure 1 |
| LD Column |
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