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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  Anti-Fluorochrome MicroBeads
  Anti-Biotin MicroBeads
  Streptavidin MicroBeads
  Monoclonal Anti-Ig MicroBeads
  Polyclonal Anti-Ig MicroBeads
  MACSiBead™ Particles
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads
Overview
Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads have been developed for indirect magnetic labeling and separation of cells labeled with primary antibodies conjugated to Cy®5, PE-Cy5, or Alexa Fluor™647. Flow cytometric analysis can be performed without any further staining of the cells.
Details
Background information
The monoclonal anti-Cy5 antibody coupled to MicroBeads recognizes Cy5, PE-Cy5, and Alexa Fluor 647. Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads may cross-react with Alexa Fluor 680, Cy5.5, Cy7, and their tandem conjugates.

Applications
Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads are especially recommended for positive selection or depletion of cells labeled with primary antibodies conjugated to PE-Cy5 tandem fluorochromes. Primary antibodies conjugated to Cy5 or Alexa Fluor 647 are also suitable for magnetic separation in combination with these MicroBeads.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Separation of CD64+ cells from human PBMCs using a PE-Cy5-conjugated CD64 antibody, Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads, a MiniMACS™ Separator, and an MS Column.
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Details
Products
Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads
- for 109 total cells
Download datasheet
130-091-395
Qty.:
 

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