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| Overview |
| Anti-Allophycocyanin (APC) MicroBeads are used for the indirect magnetic labeling and separation of cells labeled with APC-conjugated primary antibodies, peptides, or ligands. |
| Details |
Background information
The APC molecule is recognized by the monoclonal anti-APC antibody coupled to MicroBeads. Anti-APC MicroBeads are an excellent tool for positive selection or depletion of fluorescently labeled material. Subsequent flow cytometric analysis can be performed without any further staining.
Applications
Anti-APC MicroBeads can be used for the separation of any cell type stained with an APC-conjugated primary antibody. In general, a very bright fluorescence staining is obtained when using APC-conjugated primary antibodies, which makes Anti-APC MicroBeads especially suitable for the separation and subsequent detection of cells with weakly expressed markers. Anti-APC MicroBeads are also recommended for the enrichment of antigen-specific T cells after labeling with APC-conjugated peptide/MHC-tetramers. |
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| Figure 1 |
| Isolation of CD34+ cells from non-mobilized human PBMCs using CD34-APC, Anti-APC MicroBeads, a MiniMACS™ Separator, and two MS Columns. |
| PBMCs before separation |
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| CD34+ cells after separation |
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