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| Overview |
| The Anti-Phycoerythrin (PE) MultiSort Kit provides a flexible tool for the sorting of cells according to multiple surface markers. The bright fluorescence intensity of PE-conjugated antibodies makes the Anti-PE MultiSort Kit especially useful for separation and subsequent detection of cells with weakly expressed markers. |
| Details |
Detailed separation procedure
Cells are first stained with a MACS® PE-conjugated primary antibody and subsequently magnetically labeled with Anti-PE MultiSort MicroBeads. Following positive selection, the magnetic particles are removed from the cells by using the MultiSort Release Reagent. The cell population can then be magnetically labeled and sorted using a second marker of interest. This is achieved by labeling the cells directly or indirectly with MACS® MicroBeads.
Applications
The Anti-PE MultiSort Kit is recommended if a positive selection according to two cell surface antigens is desired. The kit can, for example, be used for the positive selection of rodent T cell, B cell, or progenitor cell subsets. |
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| Figure 1 |
| Separation of human CD3+CD56+ cells from PBMCs using CD56-PE, the Anti-PE MultiSort Kit, and an MS Column for the first positive selection as well as CD3 MicroBeads and an MS Column for the second positive selection. |
| PBMCs before separation |
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| Isolated CD56+ cells |
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| Isolated CD3+CD56+ cells |
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