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Anti-PE MicroBeads
Description
Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The PE molecule is recognized by the monoclonal anti-PE antibody coupled to MicroBeads, allowing the separation of the fluorescently labeled material. Subsequent flow cytometric analysis is possible without any further staining. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed.
Applications
Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Further, they have been used to sort, by MACS Technology isolated, CD4+CD45RO+ memory T cells from human adenoids according to their expression of CD62L.4
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells
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Anti-PE MicroBeads
for 109 total cells
Download data sheet
130-048-801
Qty:
 

MACS References
1. Shankar et al. (2001) Blood 96: 3094-3101.[2262]
2. Barnes et al. (2004) Eur. J. immunol. 34: 1570-1577.[3303]
3. Lucas et al. (2004) J. Virol. 78: 7284-7287.[4314]
4. Mattila et al. (2000) Int. Immunol. 12: 1235-1243.[930]
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