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| Overview |
| Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. |
| Details |
Background information
The PE molecule is recognized by the monoclonal anti-PE antibody coupled to MicroBeads, allowing the separation of the fluorescently labeled material. Subsequent flow cytometric analysis is possible without any further staining.
Applications
Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Moreover, they have been used to separate a CD62L+ subset of CD4+CD45RO+ memory T cells from human adenoids, which had been pre-sorted by MACS® Technology4. |
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| Figure 1 |
| Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns. |
| PBMCs before separation |
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| Enriched CMV-specific T cells |
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| Favorites / Prices |
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| Details |
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| Products |
| Anti-PE MicroBeads |
- for 109 total cells
Download datasheet
130-048-801
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| Anti-PE MicroBeads – lyophilized |
| Availability: (1) |
- for 109 total cells
Download datasheet
130-097-054
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| (1) Not available in Europe and the US/CAN |
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| References |
| 1. Shankar et al. (2001) Blood 96: 3094–3101. |
| 2. Barnes et al. (2004) Eur. J. Immunol. 34: 1570–1577. |
| 3. Lucas et al. (2004) J. Virol. 78: 7284–7287. |
| 4. Mattila et al. (2000) Int. Immunol. 12: 1235–1243. |
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