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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  Anti-Fluorochrome MicroBeads
  Anti-Biotin MicroBeads
  Streptavidin MicroBeads
  Monoclonal Anti-Ig MicroBeads
  Polyclonal Anti-Ig MicroBeads
  MACSiBead™ Particles
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Anti-PE MicroBeads
Overview
Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed.
Details
Background information
The PE molecule is recognized by the monoclonal anti-PE antibody coupled to MicroBeads, allowing the separation of the fluorescently labeled material. Subsequent flow cytometric analysis is possible without any further staining.

Applications
Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers1,2 or MHC Class II tetramers3. Moreover, they have been used to separate a CD62L+ subset of CD4+CD45RO+ memory T cells from human adenoids, which had been pre-sorted by MACS® Technology4.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells
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Details
Products
Anti-PE MicroBeads
- for 109 total cells
Download datasheet
130-048-801
Qty.:
 

Anti-PE MicroBeads – lyophilized
Availability: (1)
- for 109 total cells
Download datasheet
130-097-054
Qty.:
 

(1) Not available in Europe and the US/CAN
References
1. Shankar et al. (2001) Blood 96: 3094–3101.
2. Barnes et al. (2004) Eur. J. Immunol. 34: 1570–1577.
3. Lucas et al. (2004) J. Virol. 78: 7284–7287.
4. Mattila et al. (2000) Int. Immunol. 12: 1235–1243.
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