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| Description |
| Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC). The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS® Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material. |
| Applications |
| Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD+ B cells from human PBMCs1, the separation of plant protoplasts2 as well as the isolation of murine Langerhans cells from epidermal tissue3. Anti-FITC MicroBeads have also been used for the depletion of CD3+ cells from rat spleen and subsequent isolation of CD3- NK cells5 or the separation of murine naive CD8+ T cells expressing low levels of CD44 by depletion of CD44int/high cells6. |
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| Figure 1 |
| Erythrocytes from sickle cell anemia patients isolated using Annexin V-FITC and Anti-FITC MicroBeads.4 (Courtesy of Dr. Kuypers, Oakland, USA.) |
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| References |
| 1. Zan et al. (1998) J. Immunol. 161: 5217–5225.[602] |
| 2. Barth et al. (1994) J. Biochem. Biophys. Methods 29: 83–86.[6] |
| 3. Saeki et al. (2000) Eur. J. Immunol. 30: 2808–2814.[940] |
| 4. Kuypers et al. (1996) Blood 87: 1179–1187.[181] |
| 5. Mikus et al. (2001) Am. J. Respir. Cell Mol. Biol. 24: 74–82.[981] |
| 6. Schüler et al. (2004) J. Immunol. 172: 15–19.[4052] |
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