Mouse Anti-Rat Kappa MicroBeads
Description Mouse Anti-Rat Kappa MicroBeads have been developed for the positive selection or depletion of B cells according to the expression of the immunoglobulin (Ig) kappa light chain. 95% of all rat B cells express the Igκ light chain. Anti-Rat Kappa MicroBeads bind equally well to the Ig light chain allotypes Igκ-1a and Igκ-1b. B cells can be isolated from lymphoid organs and non-lymphoid tissue. Together with the Ig heavy chain, the Ig light chain is expressed on the surface of immature IgM+IgD- B cells. Furthermore, it is present on mature IgM+IgD+ B cells and memory B cells, and is down-regulated on activated B cells upon terminal differentiation into plasma cells. Applications B cells isolated with Anti-Rat Kappa MicroBeads from peripheral lymphoid organs or non-hematopoietic tissues are suitable, for example, for in vivo and in vitro studies on B cell development, differentiation, and migration. Highly pure B cell populations are also required for reliable cellular and molecular analyses, for example, of Ig rearrangement. Columns For positive selection: MS, LS, XS, or autoMACS™ Columns; for depletion: LD, CS, D, or autoMACS Columns. Further information Isolation of rat B lymphocytes [PDF; 148,1 KB]		Figure 1 Isolation of Igκ+ B cells from rat spleen using Anti-Rat Kappa MicroBeads, a MiniMACS™ Separator, and an MS Column. Spleen cells before separation Isolated Igκ+ B cells