CD45RA MicroBeads
Description Rat CD45RA MicroBeads have been developed for positive selection or depletion of B cells from blood, peripheral lymphoid organs, or from non-lymphoid tissues. The CD45RA isoform (molecular mass: 240 kDa) is B cell lineage–specific. It is neither expressed on thymocytes nor on any peripheral T cell subset. CD45RA was not detected on B cell progenitors in bone marrow. Applications Magnetically separated B cells can be used for analysis of B cell migration into non-lymphoid organs after infections, for evaluation of B cell responses after allograft transplantation, or to study the role of B cells in autoimmune diseases, such as arthritis or diabetes. Isolated B cells were used for adoptive transfer experiments to study autoimmunity and tolerance induction after xenogeneic thymus transplantation.1 Furthermore, isolated B cells were used for molecular analysis.2 Columns For positive selection: MS, LS, XS, or autoMACS™ Columns; for depletion: LD, CS, D, or autoMACS Columns.		Figure 1 Isolation of B cells from rat spleen using CD45RA MicroBeads, a MiniMACS™ Separator, and an MS Column.

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