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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  for rat cells
  T cells
  B cells
  Dendritic cells
  Antigen-presenting cells
  Neural cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD45RA MicroBeads
Overview
Rat CD45RA MicroBeads were developed for positive selection or depletion of B cells from blood, peripheral lymphoid organs, or from non-lymphoid tissues.
Details
Background information
The CD45RA isoform is B cell lineage–specific. It is neither expressed on thymocytes nor on any peripheral T cell subset. CD45RA was not detected on B cell progenitors in bone marrow.

Downstream applications
B cells separated with CD45RA MicroBeads can be used for analysis of B cell migration into non-lymphoid organs after infections, for evaluation of B cell responses after allograft transplantation, or to study the role of B cells in autoimmune diseases, such as arthritis or diabetes. Isolated B cells were used for adoptive transfer experiments to study autoimmunity and tolerance induction after xenogeneic thymus transplantation.1 Furthermore, isolated B cells were used for molecular analysis.2
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns; for depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Isolation of B cells from rat spleen using CD45RA MicroBeads, a MiniMACS™ Separator, and an MS Column.
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Details
Products
CD45RA MicroBeads, rat
- for 109 total cells
Download datasheet
130-090-494
Qty.:
 

References
1. Yan et al. (2003) J. Immunol. 170: 5936-5946.
2. Lombardi et al. (2001) J. Immunol. 166: 1635-1640.
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