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CD45R MicroBeads
Description
Rat CD45R MicroBeads have been developed for positive selection or depletion of rat B cells from bone marrow, peripheral lymphoid organs, and non-lymphoid tissue. The CD45R antigen (molecular mass: 205 kDa) is expressed throughout B cell development from the pro-B cell state on, but it is down-regulated during the differentiation of B cells into plasma cells. Expression levels of CD45R are different among distinct B cell subpopulations. Memory B cells are CD45Rlow/-. CD45R is also weakly expressed on a small subset of peripheral T cells, but is not detected on thymocytes.
Applications
B cells isolated from bone marrow or peripheral lymphoid organs can be used for in vitro or ex vivo analysis of B cell lymphopoiesis and B cell migration.
Furthermore, separation of B cells simplifies molecular analysis, for example, of immunoglobulin rearrangements.
Columns
For positive selection: MS, LS, XS, or autoMACS™ Columns; for depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Isolation of B cells from rat spleen using CD45R MicroBeads, a MiniMACS™ Separator, and an MS Column.
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Products
CD45R MicroBeads, rat
- for 109 total cells
Download data sheet
130-090-495
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