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| Overview |
| Rat CD43 MicroBeads have been developed for the isolation of untouched resting B cells from rat lymphoid tissue. |
| Details |
Background information
The CD43 antigen (leukosialin) is expressed on virtually all rat leukocytes except resting B cells.
Detailed separation procedure
After magnetic labeling with CD43 MicroBeads, non-naive B cells and other CD43+ cells are retained on the column, whereas resting B cells stay untouched and are collected in the column effluent
Downstream applications
Untouched resting B cells isolated with CD43 MicroBeads can be used for studies on B cell development and function. |
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| Figure 1 |
| Isolation of untouched B cells from rat spleen by depletion of CD43+ cells using CD43 MicroBeads, a MiniMACS™ Separator, and an MS Column. |
| A: Before separation |
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| B: Isolated resting B cells after depletion of CD43+ cells |
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