CD134 (OX40) MicroBeads
Description Rat CD134 MicroBeads have been developed for positive selection or depletion of activated CD4+ T cells from blood, lymphoid organs, and non-lymphoid tissue, such as cerebrospinal fluid and spinal cord. Expression of CD134 is restricted to activated CD4+ T cells and is up-regulated within hours after activation. CD134 and its ligand CD252 (CD134L, OX40L), which is expressed on activated T and B lymphocytes, are both members of the tumor necrosis factor receptor superfamily and were suggested to mediate the interaction between T cells and B cells. Applications Rat CD134 MicroBeads can be used for the isolation of CD4+ T cells that were activated in vivo or in vitro by a specific antigen, mitogen, or alloantigen. The magnetically enriched cells can subsequently be expanded in culture for studies on cytokine expression. Furthermore, the cells can be used for adoptive transfer or molecular analysis, such as TCR mapping. Columns For positive selection: MS, LS, XS, or autoMACSâ„¢ Columns; for depletion: LD, CS, D, or autoMACS Columns.		Figure 1 After overnight stimulation of rat spleen cells with PMA/Ionomycin and IL-4, activated T helper cells were isolated using CD134 MicroBeads, a MiniMACSâ„¢ Separator, and an MS Column. A: Spleen cells before separation B: Isolated activated CD4+ T cells