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| Description |
| Rat CD8a MicroBeads have been developed for positive selection or depletion of cytotoxic T cells from lymphoid organs, blood, or other tissues. |
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Background information
CD8a MicroBeads recognize the immunoglobulin-like domain of the CD8α chain, which is expressed as a heterodimer with CD8β on TCRα/β+ and TCRγ/δ+ cytotoxic T cells and on most thymocytes.
As a homodimer of two CD8α chains, the CD8 antigen is expressed on most NK cells,
a major fraction of intestinal intraepithelial lymphocytes, on some activated CD4+CD8+ double-positive T cells, and on CD8+ T cells from athymic rats. CD8a is also expressed by a T cell subset with regulatory functions.
Downstream applications
Thymocytes or mature T cells isolated with CD8a MicroBeads can be used for in vitro analysis1 or in vivo reconstitution2–4 of T cells, MHC restriction, tolerance induction, and cytokine expression experiments. Functionality of the isolated cells has been shown by several adoptive transfer experiments. Transfer of isolated CD4+ and CD8a+ T cells into irradiated recipients results in donor-specific acceptance to heart transplants2 or type V collagen-induced tolerance to lung allografts3. Furthermore, autoimmunity and induction of tolerance after xenogeneic thymus transplantation was studied by adoptive transfer of T cells separated by MACS® Technology.4 |
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| References |
| 1. Lombardi et al. (2001) J. Immunol. 166: 1635-1640. |
| 2. Masaaki et al. (2003) J. Immunol. 171: 204-211. |
| 3. Mizobuchi et al. (2003) J. Immunol. 171: 1140-1147. |
| 4. Yan et al. (2003) J. Immunol. 170: 5936-5946. |
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