| Description |
Rat Anti-T Cell (OX52) MicroBeads have been developed for the positive selection or depletion of rat T cells from blood, lymphoid organs, and cell suspensions of non-lymphoid tissues, such as lung or ovary.
The surface antigen recognized by the
MicroBeads is expressed in a lineage-specific manner on thymocytes and T cells. T cells expressing T cell receptor (TCR) α/β as well as TCRγ/δ are recognized. The OX52 antigen is expressed by approximately 1% of bone marrow cells, 99% of thymocytes, 60% of leukocytes in thoracic duct, 60% of lymph node cells, and 30% of splenocytes. The antigen is also weakly expressed on NK cells, which can be distinguished from T cells due to their lack of CD3 or CD5 expression. |
| Applications |
| T cells isolated with Anti-T Cell (OX52) MicroBeads can be used for in vitro and in vivo studies on T cell migration and differentiation in the context of autoimmune disease or alloreactivity. They were also used in adoptive transfer experiments to study autoimmunity or induction of tolerance after xenogeneic thymus transplantation.1 |
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| Figure 1 |
| Isolation of T cells from rat spleen using Anti-T Cell (OX52) MicroBeads, a MidiMACS™ Separator, and an LS Column. |
| A: Spleen cells before separation |
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| B: T cell-depleted fraction |
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| C: Isolated T cells |
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