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| IL-4 Secretion Assay - Detection Kit |
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| Overview |
| The IL-4 Secretion Assay was developed for the sensitive detection of human IL-4-secreting cells. |
| Details |
Background information
Interleukin-4 (IL-4) is predominantly secreted by activated CD4+ memory and effector TH2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The TH2 types of immune mechanisms can also be involved in immunologic disorders, such as IgE-mediated allergy. The IL-4 Secretion Assay (PE) can also be used for two-color cytokine analysis in combination with the IFN-γ Secretion Assay - Detection Kit (FITC) or the IFN-γ, IL-2, or IL-10 Secretion Assay - Detection Kits (APC).
Downstream applications
IL-4 Secretion Assays were used to isolate and analyze Tetanus Toxoid-specific1, allergen-specific2,5, and autoreactive7 T cells from PBMCs, as well as tumor-specific cells from T cell lines3.
The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells4, and for detection of IL-4-secreting NKT cells6.
IL-4-secreting CD8+ T cells were isolated in combination with CD8 MultiSort MicroBeads.8 |
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| Figure 1 |
| PBMCs were stimulated with 1 µg/mL SEB for 16 hours. The responding cells were stained according to secretion of IL-4 using the IL-4 Secretion Assay - Detection Kit. In the stimulated sample, 0.307% of all CD4+ T cells secreted IL-4. In the unstimulated control sample, 0.004% of all CD4+ T cells secreted IL-4. |
| A: Stimulated sample |
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| B: Unstimulated control |
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| Products |
| IL-4 Secretion Assay – Detection Kit (PE), human |
- for 100 tests with 106 total cells
Download datasheet
130-054-102
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| References |
| 1. Brosterhus et al. (1999) Eur. J. Immunol. 29: 4053-4059. |
| 2. Akdis et al. (2000) MACS&more 4: 9-10. |
| 3. Oelke et al. (2000) Scand. J. Immunol. 52: 544-549. |
| 4. Deniz et al. (2002) Eur. J. Immunol. 32: 879-884. |
| 5. Akdis et al. (2004) J. Exp. Med. 199: 1567-1575. |
| 6. Araki et al. (2002) Int. Immunol. 15: 279-288. |
| 7. Veldman et al. (2003) J. Immunol. 170: 635-642. |
| 8. Schwaiger et al. (2003) J. Immunol. 170: 613-619. |
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