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| Overview |
| The Anti-MSCA-1 antibody is used for the detection and quantification of MSCs with high proliferative capacity from bone marrow or other tissues by flow cytometry or fluorescence microscopy. |
| Details |
Background information
Clone W8B2 recognizes the mesenchymal stromal cell antigen-1 (MSCA-1). MSCA-1 was shown to be restricted to mesenchymal stromal cells (MSCs) in the CD271bright population in bone marrow1. These CD271brightCD45dim MSCs have a much higher clonogenic capacity compared to the CD271+CD45+ fraction in bone marrow1. Therefore, MSCA-1 is a suitable marker to identify MSCs with a high proliferative potential. |
| Cross-reactivity |
Antibodies against MSCA-1 have been reported2 to cross-react with
- dog
- pig
- sheep
- goat
- monkey
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| Clone | Isotype |
| W8B2 | Mouse IgG1 |
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| Figure 1 |
| Human bone marrow mononuclear cells (BM-MNCs) were stained with Anti-MSCA-1 (W8B2) antibodies conjugated to PE (A), APC (B), or Biotin (C). Cells stained with Anti-MSCA-1 (W8B2)-Biotin were additionally stained with Anti-Biotin-APC. Cells were counter-stained with CD45-FITC and analyzed by flow cytometry. |
| A |
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| B |
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| C |
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| Products |
| Anti-MSCA-1 (W8B2)-PE, human |
- for 100 tests (1)
Download datasheet
130-093-587
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| Anti-MSCA-1 (W8B2)-APC, human |
- for 100 tests (1)
Download datasheet
130-093-589
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| Anti-MSCA-1 (W8B2)-Biotin, human |
- for 100 tests (2)
Download datasheet
130-093-593
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| Anti-MSCA-1 (W8B2) pure, human |
- 100 µg in 1 mL
Download datasheet
130-093-595
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Bühring et al. (2007) Ann. NY Acad. Sci. 1106: 262–271. |
| 2. Rozemuller, H. et al. (2010) Stem Cells and Dev. 19 (12): 1911-1921. |
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