CD39 ( #130-093-506 #130-093-502 #130-093-503 #130-093-504 #130-093-505 )

CD39

Overview

The CD39 antibody can be used to identify and enumerate human or non-human primate CD39⁺ regulatory T cells and other cells expressing CD39.

Details

Background information
The CD39 antibody specifically recognizes the membrane-bound human CD39 antigen, which is expressed on an effector/memory-like subset of FoxP3⁺ regulatory T cells¹. CD39 is an ectonucleotidase and catalyzes the hydrolysis of extracellular nucleotides, for example, ATP. In concert with CD73, which is an ecto-5’-nucleotidase, this can lead to the production of adenosine. High extracellular ATP concentrations indicate tissue injury and cell death and induce various pro-inflammatory responses in immune cells.
Through its enzymatic activity, CD39 can contribute to the suppressive function of regulatory T cells, for example, by eliminating extracellular ATP or by generating adenosine, which has suppressive effects on various immune cells.

Cross-reactivity

This antibody is tested to cross-react with rhesus monkey (Macaca mulatta) cells.

Clone	Isotype
MZ18-23C8	mouse IgG1

Figure 1

Human PBMCs were stained with CD4-FITC and CD39-PE (A). Furthermore, to evaluate CD39 expression on regulatory T cells, CD4⁺ T cells were enriched using the CD4⁺ T Cell Isolation Kit II and cells were stained with CD39-APC and Anti-FoxP3-PE (B).

Figure 2

Rhesus monkey PBMCs were stained with CD39-APC and CD4-FITC and analyzed by flow cytometry.