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| Overview |
| Clone 1A8 specifically recognizes the mouse Ly-6G antigen and is suited for identification and enumeration of Ly-6G+ cells by flow cytometry or fluorescence microscopy. |
| Details |
Background information
Ly-6G is highly expressed on neutrophilic granulocytes and expression greatly increases during granulocyte maturation.1 To a lower extent, Ly-6G is also expressed during the development of monocytes. In mouse bone marrow samples, clone 1A8 primarily detects granulocytes, but not lymphocytes or erythrocytes.1 |
| Clone | Isotype |
| 1A8 | Rat IgG2a |
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| Figure 1 |
| Mouse splenocytes were stained with Anti-Ly-6G antibodies coupled to FITC (a), PE (b), or APC (c) and analyzed by flow cytometry. Cells stained with Anti-Ly-6G-Biotin (d) were additionally stained with Anti-Biotin-PE. All cells were counterstained with CD11b antibodies. |
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| B |
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| D |
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| Products |
| Anti-Ly-6G-FITC, mouse |
- for 100 tests (1)
Download datasheet
130-093-138
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| Anti-Ly-6G-PE, mouse |
- for 100 tests (1)
Download datasheet
130-093-139
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| Anti-Ly-6G-APC, mouse |
- for 100 tests (1)
Download datasheet
130-093-140
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| Anti-Ly-6G-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-093-141
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Fleming et al. (1993) J. Immunol. 151: 2399-2408. |
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