CD49d ( #130-093-283 #130-093-282 #130-093-281 #130-093-279 #130-093-280 )

CD49d

Overview

The MZ18-24A9 clone antibody is suitable for the identification and enumeration of human and non-human primate CD49d⁺ cells by flow cytometry or fluorescence microscopy.

Details

Background information
CD49d, also known as the α4 integrin chain or VLA-4α chain, is a 150 kDa cell adhesion protein that is directly involved in mononuclear leukocyte trafficking. Dimerized with β7 integrin, the resulting α4β7 integrin binds VCAM-1 (CD106), MAdCAM-1, and fibronectin to facilitate the rolling of leukocytes along vascular epithelium. In T cells, on ligation of MAdCAM-1, α4β7 integrin also provides costimulation of T cell receptor/CD3–mediated signaling.
CD49d is expressed on most mononuclear leukocytes, both in circulation or in lymphoid or other tissues, as well as on eosinophils and basophils.

Cross-reactivity

The antibody has been tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype

Figure 1

Human PBMCs were stained with CD49d antibodies coupled to FITC (A), PE (B), or APC (C). Cells stained with CD49d-Biotin (D) were stained with Anti-Biotin-APC in addition. All samples were costained with CD4 antibody conjugates and then analyzed by flow cytometry.

Figure 2

Rhesus monkey PBMCs were stained with CD49d-PE and CD4-APC and analyzed by flow cytometry

Figure 3

Cynomolgus monkey PBMCs were stained with CD49d-PE and CD4-APC and analyzed by flow cytometry.