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| Description |
The Anti-FoxP3 antibody specifically recognizes the forkhead/winged-helix transcriptional regulator FoxP3, also known as forkhead box P3, scurfin, and JM2. It is expressed predominantly in Tregs and is a major marker and functional regulator of T reg cell development and function.1,2 Mutations in the FoxP3 gene are linked to the autoimmune manifestations observed in the Scurfy mouse and humans with immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome.3,4 Studies in mice have shown that FoxP3-deficient animals lack Treg cells, whereas overexpression of the FoxP3 protein leads to profound immune suppression.
The FoxP3 Staining Buffer Set is optimized for intracellular staining of FoxP3 with clone 3G3. |
| Applications |
| The antibody is suitable for intracellular staining of FoxP3 in mouse and human cells. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| 3G3 | Mouse IgG1 |
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| Figure 1 |
| Mouse splenocytes were stained with CD4-FITC and CD25-PE or CD25-APC. Cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3-PE (A) or Anti-FoxP3-APC (B) using the FoxP3 Staining Buffer Set. Cells were analyzed by flow cytometry. |
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| B |
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| Products |
| Anti-FoxP3-PE, human and mouse |
- for 100 tests (1)
Download datasheet
130-093-014
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| Anti-FoxP3-APC, human and mouse |
- for 100 tests (1)
Download datasheet
130-093-013
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| FoxP3 Staining Buffer Set |
- for 100 tests
Download datasheet
130-093-142
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| (1) One test corresponds to fluorescent labeling of 106 cells in a total volume of 100 µL. |
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| References |
| 1. Hori et al. (2003) Science 299: 1057–1061. |
| 2. Walker et al. (2003) J. Clin. Invest. 112: 1437–1443. |
| 3. Sakaguchi et al. (1995) J. Immunol. 155: 1151–1164. |
| 4. Lundsgaard et al. (2005) Diabetes 54: 306–310. |
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